RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS DETERMINATION OF PREGABALIN AND ETORICOXIB IN PHARMACEUTICAL FORMULATIONS

Abstract

The simultaneous estimation of the Pregabalin and Etoricoxib in Tablet dosage form. Chromatogram was run through Agilent C18 150 mm (4.6 x 150mm, 5μm) Mobile phase containing Buffer 70% (Ammonium acetate ) :40% Acetonitrile was pumped through column at a flow rate of 1 ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 234.0 nm. Retention time of Pregabalin and Etoricoxib were found to be 2.233 min and 2.712 min. %RSD of the Pregabalin and Etoricoxib were and found to be 0.5 and 0.5 respectively. %Recovery was obtained as 100.06% and 99.81% for Pregabalin and Etoricoxib respectively. LOD, LOQ values obtained from regression equations of Pregabalin and Etoricoxib were 0.03, 0.09 and 0.02, 0.06 respectively. Regression equation of Pregabalin is y = 33728x + 2248.7, and y = 37618x + 3978.9 of Etoricoxib.