RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF TENEGLIPTIN AND REMOGLIFLOZIN IN BULK AND PHARMACEUTICAL DOSAGE FORM

Abstract

Simultaneous estimation of the Remogliflozin and Teneligliptin in pharmaceutical dosage form. Chromatogram was run through Discovery C18 250 x 4.6 mm, 5m. Mobile phase containing Buffer Ammonium acetate: Acetonitrile taken in the ratio 60:40 was pumped through column at a flow rate of 0.9 ml/min.. Temperature was maintained at 30°C. Optimized wavelength selected was 229 nm. Remogliflozin and Teneligliptin were eluted at 2.139 min and 2.176 min respectively. %RSD of the Remogliflozin and Teneligliptin were and found to be 0.6 and 0.7 respectively. %Recovery was obtained as 99.50% and 99.50% for Remogliflozin and Teneligliptin respectively. LOD, LOQ values obtained from regression equations of Remogliflozin and Teneligliptin were 0.11, 0.33 and 0.005, 0.014 respectively. Regression equation of Remogliflozin is y = 52813x + 14718, and y = 69817x + 586.95 of Teneligliptin.