ANALYTICAL METHOD DEVELOPMENT AND RAPID ANALYTICAL TECHNIC FOR SIMULTANEOUS ESTIMATION OF MONTELUKAST AND BILASTINE IN BULK AND PHARMACEUTICAL DOSAGE FORM BY USING RP-HPLC METHOD

Abstract

A simple, Accurate, precise method was developed for the simultaneous estimation of the Bilastine and Montelukast in bulk and pharmaceutical dosage form. Chromatogram was run through Agilent C18 150 x 4.6 mm, 5mm. Mobile phase containing Buffer :Acetonitrile taken in the ratio 70:30 was pumped through column at a flow rate of 1.0 ml/min. Buffer used in this method was 0.01N Na2Hpo4 buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 265.0 nm. Retention time of Bilastine and Montelukast were found to be 2.141 min and 2.605 min. %RSD of the Bilastine and Montelukast were and found to be 0.4% and 0.2% respectively. %Recovery was obtained as 99.47% and 99.55% for Bilastine and Montelukast respectively. LOD, LOQ values obtained from regression equations of Bilastine and Montelukast were 0.1, 0.03 and 0.03, 0.10 respectively. % Assay was obtained as 99.74% and 99.72% for Bilastine and Montelukast respectively. Regression equation of is Montelukast y = 45726x + 6306.9, y = 43360x + 810 of Bilastine.