Analytical Method Development and Validation of Macitentan and Tadalafil Using RP-HPLC in Bulk and Tablet Formulations.

Abstract

the simultaneous evaluation of Macitentan and carbidoxopa. The chromatogram was performed using Agilent (250mm 4.6mm, 5μ). A mobile phase consisting of 0.1% OPA, acetonitrile, in a 45:55 ratio was injected across the column at a flow rate of 1.0 ml/min. The temperature was kept steady at 30°C. The optimal wavelength for Macitentan and carbidogopa was 220 nm. Macitentan and carbidogpa's percentage RSDs were 0.9 and 0.8, respectively. The retention durations for Macitentan and carbidogopa were found to be 2.443 and 2.990 minutes, respectively. The Recovery was found to be 99.89% and 99.58% Respectively. The regression equation for Macitentan is y = 142567x + 5088.2, whereas that for Tadalafil is y = 203509x + 9089.6.