Development and Validation of a Novel RP-HPLC Method for Simultaneous Estimation of Budesonide and Albuterol in Bulk and Tablet Formulations

Abstract

The concurrent assessment of albuterol and Budesonide. Kromasil was used to create the chromatogram (250mm 4.6mm, 5μ). At a flow rate of 1.0 ml/min, a mobile phase made up of OPA and acetonitrile in a 45:55 ratio was injected across the column. A constant temperature of 30°C was maintained. 244 nm was the ideal wavelength for albuterol and budesonide. The percentage RSDs for bedesonide and albuterol were found to be 0.7 and 0.2, respectively, and their retention periods were 2.473 and 3.316 minutes. Budesonide and albuterol's regression models yielded LOD and LOQ values of 0.011, 0.035, and 0.01, 0.03 correspondingly. Budesonide's regression equation is y = 145694x + 700.57, while albuterol's is y = 146661x + 1161.