A Novel Rp-Hplc Method Development and Validation for Determination and Estimation of Bempedoic Acid and Ezetimibe drug with its Bulk form and Tablet Formulation

Abstract

The assessment of Ezetimibe and Bempedoic Acid at the same time. Through Sunfire (250mm 4.6mm, 5μ), the chromatogram was conducted. At a flow rate of 1.0 ml/min, a mobile phase comprising KH2PO4, acetonitrile in a 65:35 v/v ratio was injected across the column. A constant temperature of 30°C was maintained. Bempedoic Acid and Ezetimibe had an ideal wavelength of 245 nm. %RSD of Bempedoic Acid and Ezetimibe was found 1.5 and 0.6 to be Bempedoic Acid and Ezetimibe were shown to have retention times of 2.132 and 2.595 minutes, respectively. Bempedoic Acid and Ezetimibe regression equations yielded LOD and LOQ values of 0.58, 1.75, and 0.01, 0.03 correspondingly. Bempedoic Acid's regression equation is y = 7380.3x + 1190.5, while Ezetimibe is y = 12372x + 121.34.