A Validated Stability Indicating RP-HPLC Method Development for Simultaneous estimation of Cabotegravir and Rilpivirine in Pharmaceutical Dosage form

https://doi.org/10.54037/WJPS.2022.101003

Abstract

A simple, Accurate, precise method was developed for the simultaneous estimation of the Cabotegravir and Rilpivirine in pharmaceutical dosage form. Chromatogram was run through Kromasil C18 150 x 4.6 mm, 5. Mobile phase containing Buffer 0.01N Potassium dihydrogen phosphate: Acetonitrile taken in the ratio 60:40 was pumped through column at a flow rate of 1.0 ml/min. Buffer used in this method was 0.01N Kh2PO4 buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 257 nm. Retention time of Rilpivirine and Cabotegravir were found to be 2.257 min and 2.642min. %RSD of the Rilpivirine and Cabotegravir were and found to be 0.5 and 1.4 respectively. %Recovery was obtained as 100.43% and 100.13% for Rilpivirine and Cabotegravir respectively. LOD, LOQ values obtained from regression equations of Rilpivirine and Cabotegravir were 0.18, 0.54 and 0.15, 0.46 respectively. Regression equation of Cabotegravir is y = 9571.x + 4414, and  y = 5378.x + 919.7 of Rilpivirine. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in  regular Quality control test in Industries.