Antimicrobial and antioxidant activity of different extracts and polysaccharides isolated from leaves of Joannesia Princeps Vell.

Abstract

Plants have been used for thousands of years to flavour and conserve food, to treat health disorders and to prevent diseases including epidemics. The knowledge of their healing properties has been transmitted over the centuries within and among human communities. Active compounds produced during secondary vegetal metabolism are usually responsible for the biological properties of some plant species used throughout the globe for various purposes, including treatment of infectious diseases and numerous disorders. The aim of this study was to evaluate the phytochemical contents, antimicrobial & antioxidant activities of Joannesia princeps leaves extracts and isolated polysaccharides. Different extracts were prepared using soxhlet assembly and after depigmentation of leaves, leaves were undergoing for the isolation of polysaccharides. All four extracts were tested for the presence of phytoconstituents and all four extract and isolated polysaccharides were also evaluated for antimicrobial and antioxidant effects. From antimicrobial activity, methanol extract showed maximum inhibition zone at a concentration of 200 mg/ml. The concentration of 500 µg/ml of methanol extract showed maximum antioxidant activity 83.69 % in comparison to other extract. CWP and HWP polysaccharides showed a significant antimicrobial and antioxidant effects. Methanol and acetone were richest in presence of phytoconstituents.